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Peptide Synthesis Guide

Peptide Synthesis Guide

3 Documents Available

PDF

Solid Phase Synthesis Overview

Introduction to Fmoc and Boc SPPS strategies, resin selection, and coupling reagent choice.

Size: 2.4 MB
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PDF

Custom Peptide Order Guide

Step-by-step instructions for submitting custom peptide sequences, scale, and purity requirements.

Size: 1.8 MB
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PDF

Sequence Notation Standards

Accepted single-letter and three-letter amino acid codes, modification notation, and special residues.

Size: 1.2 MB
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Modifications & Conjugations

Modifications & Conjugations

4 Documents Available

PDF

N- & C-Terminal Modifications

Available acetylation, amidation, biotinylation, and fluorescent label options.

Size: 3.1 MB
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PDF

PEGylation Services

Guidelines for PEG chain selection, attachment sites, and impact on solubility and half-life.

Size: 2.7 MB
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PDF

Stapled & Cyclic Peptides

Design considerations for lactam bridges, disulfide cyclization, and hydrocarbon stapling.

Size: 4.2 MB
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PDF

Isotope Labeling Guide

Stable isotope (¹³C, ¹⁵N, ²H) incorporation for quantitative mass spectrometry workflows.

Size: 2.9 MB
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Analytical & QC Documentation

Analytical & QC Documentation

4 Documents Available

PDF

HPLC Purity Analysis

Methodology for reverse-phase HPLC purity assessment and interpretation of chromatograms.

Size: 3.5 MB
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PDF

Mass Spectrometry Verification

ESI-MS and MALDI-TOF protocols used to confirm molecular weight and sequence integrity.

Size: 4.1 MB
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PDF

Certificate of Analysis (CoA)

Sample CoA template showing purity, mass confirmation, appearance, and storage conditions.

Size: 1.4 MB
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PDF

Amino Acid Analysis

Hydrolysis and derivatization procedures for quantitative amino acid composition analysis.

Size: 2.6 MB
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GMP & Regulatory Compliance

GMP & Regulatory Compliance

4 Documents Available

PDF

GMP Manufacturing Overview

Facility standards, batch record documentation, and quality system requirements for GMP peptides.

Size: 5.3 MB
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PDF

ICH Q7 Compliance Summary

How our production processes align with ICH Q7 guidelines for active pharmaceutical ingredients.

Size: 3.8 MB
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PDF

FDA DMF Filing Support

Documentation support for Drug Master File submissions including peptide API characterization.

Size: 2.3 MB
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PDF

Impurity Profiling Standards

Methods for identifying and quantifying related substances, residual solvents, and counterions.

Size: 4.7 MB
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Handling & Storage

Handling & Storage

4 Documents Available

PDF

Lyophilized Peptide Handling

Best practices for reconstitution, aliquoting, and avoiding freeze-thaw degradation.

Size: 2.1 MB
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PDF

Solubility Troubleshooting

Strategies for dissolving difficult peptides including hydrophobic, charged, and aggregation-prone sequences.

Size: 1.9 MB
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PDF

Long-Term Storage Conditions

Temperature, light, and humidity recommendations for maintaining peptide stability over time.

Size: 1.5 MB
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PDF

Cold Chain Shipping Protocol

Dry ice and cold pack packaging standards for temperature-sensitive peptide shipments.

Size: 2.4 MB
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Order Forms & Templates

Order Forms & Templates

4 Documents Available

DOC

Custom Synthesis Request Form

Downloadable order form for custom peptide sequences, scale, purity grade, and modification requests.

Size: 0.4 MB
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XLS

Bulk Order Quotation Template

Spreadsheet template for submitting multi-peptide library or large-scale production quote requests.

Size: 0.3 MB
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PDF

NDA / Confidentiality Agreement

Standard non-disclosure agreement for proprietary sequence and project confidentiality.

Size: 0.5 MB
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PDF

Incoming Sample Submission Form

Use when sending reference standards or raw materials for analysis or custom conjugation work.

Size: 0.4 MB
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Support

Frequently Asked Questions

Quick answers to common questions about our documentation and resources.

What purity grades do you offer for custom peptides?

We offer crude, >70%, >80%, >90%, >95%, and >98% purity grades by HPLC. Research-grade applications typically use >90%, while therapeutic or analytical reference standards require >95% or >98%. Each order ships with a Certificate of Analysis confirming purity and mass.

What is the typical turnaround time for a custom peptide order?

Standard turnaround is 5–10 business days for most research-grade peptides up to 30 residues at >95% purity. Longer sequences, difficult sequences, or GMP-grade orders may require 3–6 weeks. Rush synthesis options are available — contact us for availability.

Do you synthesize peptides with non-standard or unusual amino acids?

Yes. We routinely incorporate D-amino acids, N-methylated residues, phosphorylated residues (pSer, pThr, pTyr), unnatural amino acids, and a range of fluorescent labels. Please include full modification details when submitting your sequence.

How should I store my lyophilized peptide upon receipt?

Lyophilized peptides should be stored at -20°C in a dry environment, away from light. Avoid repeated freeze-thaw cycles by aliquoting upon first reconstitution. Hydrophilic peptides are generally stable at -20°C for 12+ months; hydrophobic or cysteine-containing peptides may require -80°C for long-term storage.

Can you provide GMP-grade peptides for clinical or pharmaceutical use?

Yes. We offer GMP synthesis services compliant with ICH Q7 guidelines, including full batch documentation, impurity profiling, residual solvent analysis, and support for FDA Drug Master File (DMF) submissions. Contact our team to discuss your regulatory requirements.

What file format should I use to submit my peptide sequence?

Sequences can be submitted using standard single-letter or three-letter amino acid codes. Modifications should be noted in brackets adjacent to the relevant residue (e.g., K[Biotin], pS for phosphoserine). Download our Custom Synthesis Request Form from the Order Forms section for a guided submission template.

Need More Help?

Our team is here to assist you with any questions or support you need.

Have a Synthesis Question?

Our peptide specialists are available to advise on sequence design, modification strategy, scale-up, and analytical requirements.

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Synthesis Protocols & Videos

Access step-by-step video walkthroughs covering reconstitution, HPLC interpretation, and handling difficult sequences.

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New Products & Updates

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